François Huber was only fifteen years old when he began to suffer from a disease which gradually resulted in total blindness; but, with the aid of his wife, Marie Aimée Lullin, and of his servant, François Burnens, he was able to carry out investigations that laid the foundations of a scientific knowledge of the life history of the honey bee. His Nouvelles Observations sur les Abeilles was published at Geneva in 1792 (Eng. trans., 1806). A second volume of work published along with the first came out in 1814 which covered many more subjects including the construction of comb and experiments on the respiration of bees.